BIOPHYSICS OF MOLECULES AND MOLECULAR MACHINES

PHYSICS 146A/230A

Quarter: Fall Quarter 2006


Goals of the course:

This course has three main goals. It will introduce students to basic concepts and language of biology that are necessary to engage in a meaningful dialogue with biological researchers. Emphasis will be on overarching concepts, challenges, and principles rather than cataloguing details. The student will see several examples of biological problems and approaches to their solution that require a true interplay between physics, computation, and biology (not just adding numbers, equations or standard physics approaches to biological questions). All examples are chosen such that molecular information is important, but also such that an understanding of biological function requires description of the system and its properties. Examples include: regulation of gene transcription in bacteria, transport of molecules by free diffusion and across membranes, bacterial chemotaxis, photosynthetic systems and transduction of nerve impulses. The third goal is to give the student a brief overview of state-of-the art experimental and modeling approaches as a starting point for further studies.


Materials

Lecture materials will be posted on this webpage. There is no one book that covers the scope and goals of this course. We will draw from Alberts et al: Molecular Biology of the Cell for some of the background biology, and from Philip Nelson: Biological Physics, Howard Berg: Random Walks in Biology, Jonathon Howard: Mechanics of Motor Proteins and the Cytoskeleton for some of the physics descriptions.


Grading 

 

Grades will be determined based on completion of homework assignments (60%), class participation (10%) and the final examination (30%). There will be no midterm exam. Homework sets stimulate quantitative analysis of the concepts discussed in the lecture and can involve small programming effort. The final exam will test mostly for acquired knowledge.

 


 

Schedule and Lecture Notes

Week 1 (Sep 25-Sep29)  What are the building blocks? (aka Review of Molecular Microbiology)                                                                                                 

Mon, Sep 25: genomic information, central dogma, notes, ppt

Wed, Sep 27: flow of genomic information; proteins, cells, notes

Fri, Sep 29: DNA-transcription factors in bacteria: biological facts, notes

Week 2 (Oct 2-Oct 7)  Example: Transcription regulation through protein DNA-interactions (lac-repressor)

Mon, Oct 2: DNA-TF interactions I, notes

Wed, Oct 4: DNA-TF interactions II, notes    

Fri, Oct 6: DNA-TF interactions III, notes

Week 3 (Oct 9-Oct 13)   How do things move in cells?

Mon, Oct 9: free diffusion, first mean passage times, notes

Wed, Oct 11: diffusion to multiple absorbers, glimpse into more general statistical mechanics, notes      

Fri, Oct 13: lecture cancelled

Week 4 (Oct 16-Oct 20)   Molecular Level information: X-ray crystallography, NMR                                                                      

Mon, Oct 16: DNA-TF interactions with dynamics, notes

Wed, Oct 18: X-ray crystallography, slides

Fri, Oct 20: Structure determination with NMR

Week 5 (Oct 23-Oct 27)   Electrical energy in cells

Biological membranes, ion transport, ion channels, ion pumps

notes

Week 6 (Oct 30-Nov 3)   How do we model biological systems?

Mon, Oct 30: Molecular Dynamics, notes

Wed, Nov 1: Brownian Dynamics, Monte-Carlo, Poisson Boltzmann

Fri, Nov 3: Sampling: Jarzynski equality, autocorrelation, notes

Week 7 (Nov 6-Nov10)   Signaling

Mon, Nov 6: Signaling overview, notes

Wed, Nov 8: Bacterial chemotaxis: slides

Fri, Nov 10: Bacterial chemotaxis: Leibler paper

Week 8 (Nov 13-Nov17)   Control Theory

Mon, Nov 13: no lecture

Wed, Nov 15: Control theory I, notes

Fri, Nov 17: Control theory II, notes

Week 9 (Nov 20-Nov24)   Example: Photosynthesis: harvesting sun light and bioelectronics                                                                   

slides

Week 10 (Nov 27-Dec 1)